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31.
Junji Tsuruta Katsunori Sugisaki Arthur M. Dannenberg Jr Teizo Yoshimura Yasuharu Abe Phoebe Mounts 《Inflammation》1996,20(3):293-318
Developing and healing dermal inflammatory lesions were produced in rabbits by the topical application of dilute sulfur mustard (SM),9 the military vesicant. In tissue sections of such lesions, cells containing the mRNA of important cytokines were identified with in situ hydridization techniques. These cytokines were neutrophil attractant/activation protein-1 (NAP-1 (also called IL-8)), monocyte chemoattractant (activating) protein 1 (MCP-1), interleukin 1 (beta) (IL-1 (beta)), and GRO (a growth factor and chemokine). Mononuclear cells (mainly macrophages and activated fibroblasts) contained the mRNA of all four of these cytokines. A higher percentage of cytokine-producing mononuclear cells (macrophages and activated fibroblasts) was present in lesions at 2 days (their peak size) than at 6 days, when they were almost healed. Granulocytes emigrated from the bloodstream, passed through the lesions, and were the major constituent of the protective crust. This sequence correlated with the distribution of cells able to produce NAP-1: At 2 days and 6 days, the mononuclears that contained messenger RNA for this granulocyte chemoattractant were found mainly in the upper part of the dermis. At 2 days and 6 days, cells containing the mRNA of IL-1, a primary cytokine, were also found predominantly in the upper dermis, i.e., nearest the site of injury. In contrast, mononuclears containing the mRNA of MCP-1 (a monocyte chemoattractant), and the mRNA of GRO (a granulocyte chemoattractant) were more equally distributed throughout the dermis. SM stimulated hair follicle epithelial cells to up-regulate GRO mRNA and, to a lesser degree, NAP-1 mRNA. Apparently, the irritation produced by SM directly or indirectly induces such epithelial cells to manufacture these growth factors. In the rabbit, hair follicles are known to be the main source of new epithelial cells after the covering epithelium has been destroyed. Therefore, GRO is probably a major autocrine-paracrine stimulus for such repair. A brief review of the role of cytokines in dermal inflammation is presented.Abbreviations SM
Sulfur mustard: bis(2-chloroethyl)sulfide
- GM-CSF
Granulocyte-Macrophage Colony Stimulating Factor
- GRO
A member of the CXC subfamily of chemokines that promotes the multiplication of cells, formerly called melanoma growth stimulating activity (MGSA)
- IFN
(gamma)-Interferon-gamma
- IL-1
Interleukin 1
- IL-8
Interleukin 8 (same as NAP-1)-a CXC chemokine
- MCP-1
Monocy te Chemoattractant (Activating) Protein-1-a C-C chemokine
- NAP-1
Neutrophil Attractant/Activating Protein-1 (same as IL-8)-a C-X-C chemokine
- TGF (beta)
Transforming Growth Factor (beta)
- TNF (alpha)
Tumor Necrosis Factor (alpha)
- EDTA
Ethylenediamine tetraacetate
- DEPC
Diethylpyrocarbonate
- PBS
Phosphate-buffered saline solution
- PGE2
Prostaglandin E2
- PGI2
Prostaglandin I2 (prostacyclin)
- SSC
Sodium chloride-sodium citrate solution
On leave of absence from the Institute for Medical Immunology, Kumamoto University School of Medicine, Kumamoto, Japan.On leave of absence from the Department of Internal Medicine, Oita Medical University, Oita, Japan. 相似文献
32.
33.
Mitsui A Hamuro J Nakamura H Kondo N Hirabayashi Y Ishizaki-Koizumi S Hirakawa T Inoue T Yodoi J 《Antioxidants & redox signaling》2002,4(4):693-696
Transgenic (Tg) mice overexpressing human thioredoxin (TRX), a small redox-active protein, were produced to investigate the role of the protein in a variety of stresses. Bone marrow cells from TRX-Tg mice were more resistant to ultraviolet C-induced cytocide compared with those from wild type (WT) C57BL/6 mice. TRX-Tg mice exhibited extended median and maximum life spans compared with WT mice. Telomerase activity in spleen tissues in TRX-Tg mice was higher than that in WT mice. These results suggest that overexpression of TRX results in resistance against oxidative stress and a possible extension of life span without apparent abnormality in mammals. 相似文献
34.
Solid phase activation of alternative pathway of complement by beta-1,3-glucans and its possible role for tumour regressing activity. 总被引:2,自引:0,他引:2 下载免费PDF全文
The efficiency of some anti-tumour polysaccharides, such as lentinan, pachyman, pachymaran, carboxymethylpachymaran and hydroxyethylpachyman to trigger the alternative pathway of complement activation (APC) was investigated in detail, in order to clarify whether solid phase activation of APC by these polysaccharides will occur or not. From the eight polysaccharides tested, all were found to be potent activators of the alternative pathway except for carboxymethylpachymaran, regardless of their potency of inhibition of sarcoma 180 (S 180) transplanted in mice. This activation was observed both for the insoluble as well as the soluble part of the same polysaccharide. The turnover of C3, C5 and factor B showed no difference among these seven active polysaccharides. The polysaccharide particles (PX), isolated after treatment with C4d GPS showed prominent C3 consuming activity, which disappeared during prolonged incubation at 37 degrees. The activity of the decayed enzyme could be regenerated by treatment with the purified factor B. The stability of the particulate enzyme PX was comparable to the zymosan-complex (ZX) previously described. 相似文献
35.
Euy Kyun Shin Fumihiko Matsuda Junji Fujikura Takashi Akamizu Hideo Sugawa Toru Mori Tasuku Honjo 《European journal of immunology》1993,23(9):2365-2367
In an Epstein-Barr virus-transformed human B cell line we found an unusual immunoglobulin heavy chain gene rearrangement. Restriction mapping and sequencing analysis led us to conclude that VH-D and D-JH recombination took place in a single allele. Both VH-D and D-JH complexes still had their recombination signal sequences adjacent and the DNA sandwiched by these two complexes retained a germ line configuration, suggesting the potential for a secondary rearrangement resulting in a VH-D(-D)-JH formation. With this finding, we propose a novel pathway, in which the VH-D complex is an intermediate in the formation of a functional VH exon. 相似文献
36.
Vidar Wendel-Hansen Michèle Rivière Masatsune Uno Irma Jansson Josiane Szpirer M. Quamrul Islam Göran Levan George Klein Junji Yodoi Anders Rosén Claude Szpirer 《Somatic Cell and Molecular Genetics》1990,16(3):283-286
Using Southern blot analysis of DNAs from human×rodent cell hybrids, we have mapped the CD23 leukocyte antigen gene (FCE2) to human chromosome 19. 相似文献
37.
Kazutoshi Ono Yoshiyuki Yamanaga Kyousuke Yamamoto Shun-Ichi Koga Junji Nishimura Hajime Nawata 《Journal of clinical immunology》1996,16(1):41-45
Previously we proposed a new analysis of natural killing activity, for comparison, employing an individual effector/ target cell ratio according to the peripheral number of effector cells. Using this analysis, we studied natural killing activities in chronic liver diseases and hepatocellular carcinoma (HCC). The activity in chronic persistent hepatitis remained nearly at the level of the nonactivated state, but that was significantly elevated in chronic active hepatitis. The activity in liver cirrhosis (LC) of Child's A or B grade was at the level of a nonactivated or reduced-activity state, while LC patients with impaired general conditions showed significantly elevated activities. In HCC, each of which was accompanied by LC in our cases, the activity appeared to be associated with the progression of HCC. Thus, natural killing activity showed a close relationship with the condition of chronic liver diseases. 相似文献
38.
Junji Furukawa Jitsuo Kiji Hisatoshi Konishi Kazuyoshi Yamamoto Shin-Ichi Mitani Susumu Yoshikawa 《Macromolecular chemistry and physics.》1973,174(1):65-71
The behavior of hydridonickel coordination compounds as catalysts for the oligomerization and polymerization of butadiene in various solvents was studied. In the presence of alcohol bis(tricyclohexylphosphine)chlorohydridonickel ( 4 , X = Cl) (HNiCl[P(C6H11)3]2) catalyzes the linear dimerization. With hydridotetrakis(phosphite)nickel(1+) ( 2 ) ([HNi{P(OR)3}4]+), which is prepared from tetrakis(phosphite)nickel ( 1 ) (Ni[P(OR)3]4) and trifluoroacetic acid, dimerization occurs in sec-alcohol but there is no reaction in tert-alcohol. The main product is 2-methylenevinylcyclopentane ( 8 ). The other products are 4-vinylcyclohexene ( 10 ), 1,5-cyclooctadiene ( 5 ), 1,3,7-octatriene ( 7 ) and 1,3,6-octatriene ( 9 ). The hydridonickel coordination compound, prepared with inorganic acids, does not afford the dimers but the 1,4-trans polymer. 相似文献
39.
Suppression of UV-induced mutations by wild-type p53 protein in human osteosarcoma cells 总被引:2,自引:0,他引:2
We have examined whether the tumour suppressor p53 protein suppressedUV-induced mutations in the hypoxathine-guanine phosphoribosyltransferase (HPRT) gene and in the supF gene of the shuttlevector plasmid pMY189. We used human osteosarcoma Saos-LP12cells, in which wild type (wt) p53 protein was induced by treatmentwith isopopyl-(ß-D-thiogalactopyranoside. The inductionof wt p53 protein suppressed UV-induced mutations but not spontaneousmutations in the HPRT gene. The frequency of UV-induced mutationsinduced by UV-irradiation of the plasmid was also significantlylower in cells with induced wt p53 protein than in the uninducedcells. In addition, we found that frequency of G : C to A :T transition mutations which occurred at the 3' base pair ofdipyrimidine sites were significantly lower in the cells withinduced wt p53 protein than in the uninduced cells. These findingssuggestthat wt p53 protein may play roles in modulating DNArepair pathway, resulting in the suppression of UV-induced mutations.
1To whom correspondence should be addressed 相似文献
40.
Katsuaki Ito Erika Shimomura Takahiro Iwanaga Mitsuya Shiraishi Kazutoshi Shindo Junji Nakamura Hiromitsu Nagumo Minoru Seto Yasuharu Sasaki† Yoh Takuwa‡ 《The Journal of physiology》2003,546(3):823-836
Although the prostate gland is a rich source of α1-adreno- (α1-AR) and m1-cholino receptors (m1-AChR), the membrane processes associated with their activation in glandular epithelial cells is poorly understood. We used the whole-cell patch-clamp technique to show that the agonists of the respective receptors, phenylephrine (PHE) and carbachol (CCh), activate cationic membrane currents in lymph node carcinoma of the prostate (LNCaP) human prostate cancer epithelial cells, which are not dependent on the filling status of intracellular IP3 -sensitive Ca2+ stores, but directly gated by diacylglycerol (DAG), as evidenced by the ability of its membrane permeable analogue, OAG, to mimic the effects of the agonists. The underlying cationic channels are characterized by the weak field-strength Eisenman IV permeability sequence for monovalent cations ( P K (25) > P Cs (4.6) > P Li (1.4) > P Na (1.0)), and the following permeability sequence for divalent cations: P Ca (1.0) > P Mg (0.74) > P Ba (0.6) > P Sr (0.36) > P Mn (0.3). They are 4.3 times more permeable to Ca2+ than Na+ and more sensitive to the inhibitor 2-APB than SK&F 96365. RT-PCR analysis shows that DAG-gated members of the transient receptor potential (TRP) channel family, including TRPC1 and TRPC3, are present in LNCaP cells. We conclude that, in prostate cancer epithelial cells, α1-ARs and m1-AChRs are functionally coupled to Ca2+ -permeable DAG-gated cationic channels, for which TRPC1 and TRPC3 are the most likely candidates. 相似文献